Towards sex identification of Asian Palmyra palm (Borassus flabelliferL.) by DNA fingerprinting, suppression subtractive hybridization andde novotranscriptome sequencing
نویسندگان
چکیده
منابع مشابه
Suppression subtractive hybridization.
Suppression subtractive hybridization (SSH) is a widely used method for separating DNA molecules that distinguish two closely related DNA samples. Two of the main SSH applications are cDNA subtraction and genomic DNA subtraction. In fact, SSH is one of the most powerful and popular methods for generating subtracted cDNA or genomic DNA libraries. The SSH method is based on a suppression PCR effe...
متن کاملIdentification of Salmonella pullorum genomic sequences using suppression subtractive hybridization.
Pullorum disease affecting poultry is caused by Salmonella enterica serovar Pullorum and results in severe economic loss every year, especially in countries with a developing poultry industry. The pathogenesis of S. Pullorum is not yet well defined, as the specific virulence factors still need to be identified. Thus, to isolate specific DNA fragments belonging to S. Pullorum, this study used su...
متن کاملIdentification of Differential Genes by Suppression Subtractive Hybridization: VI. Differential Hybridization with Tester and Driver DNA Probes.
INTRODUCTIONSuppression subtractive hybridization (SSH) is one of the most powerful and popular methods for generating subtracted cDNA or genomic DNA libraries. This technique can be used to compare two mRNA populations and obtain cDNAs representing genes that are either overexpressed or exclusively expressed in one population as compared to another. It can also be used for comparison of genomi...
متن کاملPosters IDENTIFICATION OF SEX SPECIFIC DNA REGIONS IN THE SNAKE GENOME USING A SUBTRACTIVE HYBRIDIZATION TECHNIQUE
Karyotypic studies have shown that a ZZ/ZW sex chromosome system is used by snakes, which chromosomally resembles the ZZ/ZW system used by birds. However genetic studies have shown that SOX 3; the sex determining gene in mammals and DMRT1; which is the hypothesised sex determining gene in birds, are both located on autosomal chromosomes in the snake. Therefore it has been suggested that the sna...
متن کاملIdentification of Differential Genes by Suppression Subtractive Hybridization: III. PCR Amplification of Differentially Presented DNAs.
INTRODUCTIONSuppression subtractive hybridization (SSH) is one of the most powerful and popular methods for generating subtracted cDNA or genomic DNA libraries. This technique can be used to compare two mRNA populations and obtain cDNAs representing genes that are either overexpressed or exclusively expressed in one population as compared to another. It can also be used for comparison of genomi...
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ژورنال
عنوان ژورنال: PeerJ
سال: 2019
ISSN: 2167-8359
DOI: 10.7717/peerj.7268